1st- 3rd generation – probiotics

4st generation – probiotics contain solitary cells

New generation of probiotics

New generation of probiotics is already the 4th generation of probiotics. Their structure is fundamentally different from the previous three generations. The previous generations contain separate, solitary cells of probiotics while the probiotics of the 4th generation bring pure bacterial culture in the form of a biofilm, i.e. in a structure similar to that one in which they occur on intestinal mucosa. Probiotic bacteria are produced and cultivated to create biofilm in order to be most functional and natural. The bacteria cultivation that results as a biofilm is a patent-protected technology.

Features of probiotics of 4th generation:

  • their structure is very close to real state in which they occur on intestinal mucosa
  • their genes are prepared to adhere quickly on intestinal epithelium
  • they are stable
  • they are more resistant to acidic pH of stomach
  • they are more resistant to selected antibiotics
  • they are more resistant to bile acid

1st – 3rd generation of probiotics and their limits

In approximately 15 years that probiotics have been on the market, they highly improved. To summarize briefly the development of probiotics, we can divide them into 3 generations:

  • 1st generation – probiotics whose bacterial cells are not processed
  • 2nd generation – enteric-coated, protected against acidic pH of the stomach
  • 3rd generation – encapsuled probiotics with elevated stability

All probiotic preparations of the 1st –3rd generation contain solitary cells.

However, the structure of bacteria in our intestine is different. If we study intestinal mucosa, we find bacteria in the form of a biofilm.

For the right function of probiotics it is necessary that they adhere to intestinal mucosa and form a so-called biofilm.

And this could be a problem. Bacteria that are cultivated as solitary cells, must be radically reprogrammed so that they are able to form a biofilm. It this does not happen, probiotic bacteria of 1st-3rd generation pass through digestive tract with no benefit.

Comparison of biofilm probiotics and commonly cultivated probiotics:

Monitored parameter Laktobacillus acidophilus as a biofilm Laktobacillus acidophilus as solitary cells
Resistance to bile in time high low
Resistance to low pH in time high low
Adhesion on surface high low
Resistance to selected antibiotics high low

The development of new generation of probiotic cultures is a result of our many years´ research in probiotics. Our newly developed product brings an absolute revolution on the market with probiotic preparations.


Dietary supplement ProbioLact is so far the only
representative of biofilm probiotics on the market. Unique probiotic cells are present in the form of a biofilm. The biofilm is a natural and coherent layer of bacteria that create protective and stimulative barrier. This form of bacterium existence is essential for the right function of intestinal microflora. Dietary supplement ProbioLact brings an absolute revolution on the market with probiotic preparations.




ProbioLact contains:

Biofilm probiotics
Prebiotics (to stimulate the growth of beneficial microflora)
Vitamin C (for immunity and metabolism)
Special enteric-coated capsule (protection against acidic environment of stomach)

Active ingredients in 1 capsule:

Active ingredients mg /1 cps %RDI*
Lactobacillus acidophilus, 
Lactobacillus sporognes,
Bifidobacterium bifidum,
Bifidobacterium breve
Lactobacillus acidophilus hard biofilm
5 x 109 CFU** -
Vitamín C 40 50%

* RDI – Recommended daily intake – it is not determined
**CFU - colony-forming unit